ENDONANO – Quantitative detection of bacterial endotoxin by novel nanotechnological approaches

ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory
and safety issue, i.e., the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug
development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to
interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new
concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular
beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions.
The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing
inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal
nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation
and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and
validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will
include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific
events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with
strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for
modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and
one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs).

Deadline 30 December 2022

Programme HORIZON 2020 MSCA-ITN-EID – European Industrial Doctorates

Institute ISA – Institute of food sciences

Contact Sabato D’auria

Link https://cordis.europa.eu/project/rcn/217889/factsheet/en

Area Difesa delle produzioni e sicurezza della catena agroalimentare

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